standard-title Gender Selection (TruGender)

Gender Selection (TruGender)

TruGender is a highly accurate genetic test used to identify the gender of IVF embryos prior to implantation. Based on a robust quantitative Polymerase Chain Reaction (qPCR) method, TruGender relies on highly optimized DNA probes designed to reveal the copy number of the indicated chromosomes. In addition to distinguishing male (XY) and female (XX) embryos, TruGender can identify abnormal sex chromosome conditions such as Turner syndrome (XO) and Klinefelter syndrome (XXY).

What are the benefits of TruGender?

TruGender was developed as an alternative to the traditional cytogenetic FISH-based protocol. As a molecular genetics method, TruGender is more sensitive and accurate. Interpretation of TruGender test results are automated and objective, in contrast to the visual assessment of a laboratory scientist required by FISH.

Additionally, TruGender provides quality results at any stage of embryo development where FISH is limited to blastomere (day 3) biopsy. Trophectoderm (day 5) biopsy not only provides better PGS results, but is also less traumatic for the embryo. Because the TruGender workflow can be completed in just 6 hours, fresh transfers are possible even with day 5 biopsy.




Low-risk couples using IVF for the sole purpose of selecting the gender of their child for family balancing or medical necessity will benefit most from the TruGender PGS option. In these cases, comprehensive 24 chromosome screening is an unnecessary cost for the patients and TruGender may be a more affordable option. Additionally, the accuracy of sex chromosome copy number is improved for TruGender compared to the array-based CGH-24 test.

What are the advantages of TruGender testing at Reprogene?

  • Highly precise copy number determination of chromosomes X and Y.
  • Robust genetic test developed with rigorous in-house validation
  • A turn-around-time of 6 hours allows for fresh transfer even with day 5 biopsy
  • More accurate gender determination than FISH
  • Simple workflow leads to low rate of failure
  • Lower cost option compared to comprehensive chromosome screening
  • State of the art genetics facility with fully equipped advanced technologies
  • Personalized/customized services to meet your center and patients’ needs
  • Flexible service options to immediately implement procedures
  • Best price without compromising service quality
  • Available 24 hours, 7 days a week, including holidays
  • Accurate, comprehensive, and fast turnaround time for test results
  • CLIA and State Certified

There are many reasons why people may choose to have a girl or a boy. In some circumstances,choosing the gender is necessary for the following:

Gender selection for family balancing

Sex selection, also known as gender selection, is a method of fertility treatment that allows parents to know the sex of their offspring prior to implantation. Sex selection is often chosen by couples to balance the sex of their family, referred to as “family balancing.” For example, a couple with three boys may desire to select the sex of their fourth child to have a baby girl.

In some countries, gender selection is considered unethical and in the European Union, unlawful. Proponents against gender selection argue that it can lead to discrimination or population unbalance. In the United States, this has been disproven as most families in the US use gender selection as a means to balance the gender of their offspring.

Gender selection for X-linked disorders

X-linked disorders (e.g., Haemophilia A, Alport’s Syndrome and OCT deficiency) are caused by mutations on the X chromosome. As the X chromosome is one of the sex chromosomes,the possibility of a particular embryo being affected is dependent upon whether the embryo is male or female.

This type of testing involves identifying the sex of the embryo rather than the particular genetic condition. For most conditions, female embryos (which can be normal or carriers of the condition) will be selected for transfer. Male embryos (which can be carriers or affected by the conditions) will not be transferred and could be frozen if they reach the appropriate developmental stage.